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• Anti-Mouse MIF Pre-coated 96 well Strip Microplate
• Mouse MIF Detection Antibody
• Mouse MIF Standard
• Avidin-HRP
• Assay Buffer B
• Wash Buffer (20X)
• Substrate Solution F
• Stop Solution
• Plate Sealers

Macrophage migration inhibitory factor (MIF), also known as glycosylation inhibiting factor (GIF), is a pituitary hormone and a macrophage- and T cell-derived cytokine that plays an important role in the endocrine and immune systems. MIF consists of 115 amino acids and has a molecular weight of 12.5 kD. Mouse MIF shares most of its identity with human (90%), rat (99%), and cattle (88%) MIFs, and a moderate percentage of its identity with MIFs from a wide variety of other species. It exists in monomeric, dimeric, and trimeric forms of identical protomers.

MIF is active as a pro-inflammatory regulator and upregulates multiple cytokines that are vital to combating infections in vivo, including tumor-necrosis factor (TNF), interferon-γ (IFN-γ), and interleukin-12 (IL-12). It is known to maintain cell survival and promote pro-inflammatory immune responses. MIF also activates the ERK1/ERK2 signaling pathway, which inhibits the immunosuppressive effects of glucocorticoids. MIF also plays a critical role in superantigen-induced toxic shock. It upregulates several factors that enhance the growth and invasiveness of gram-negative bacteria. MIF can enhance many inflammatory diseases, such as acute respiratory distress syndrome, arthritis, atopic dermatitis, allograft rejection, glomerulonephritis, inflammatory bowel diseases, asthma, and atherosclerosis. Known receptors for MIF include CXCR2, CXCR4, CD44, and CD74.

The LEGEND MAX™ Mouse MIF ELISA kit is a ready-to-use Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse MIF antibody. The Detection Antibody is a biotinylated goat polyclonal anti-mouse MIF antibody. This assay is specifically designed to quantify mouse MIF from serum, plasma, cell culture supernatant, and cell lysate. This kit is analytically validated with prepared reagents.