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QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒

更新時間： 2023-07-26
產(chǎn)品型號： 12381
簡單描述
QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒
EndoFree Plasmid Kits基于陰離子交換技術，可快速制備不含內(nèi)毒素的質(zhì)粒DNA。QIAfilter Cartridges過濾快速澄清裂解液。制備的DNA純度超過兩次CsCl梯度離心法所得DNA的純度，適合超轉(zhuǎn)染級純的應用。EndoFree Plasmid Buffer Set可用于制備10次m

詳細介紹

QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒

純化得到至多10 mg的無內(nèi)毒素高轉(zhuǎn)染級純質(zhì)?；蚩滤官|(zhì)粒DNA

純化得到的DNA內(nèi)毒素水平低于0.1 EU/µg
純化流程快，同時去除內(nèi)毒素
獲得高產(chǎn)量、高拷貝質(zhì)粒DNA
使用LyseBlue，獲得的裂解和高產(chǎn)量的DNA

QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒

質(zhì)粒純化方法與轉(zhuǎn)染效率。

使用兩種方法獨立制備pRSVcat，分別使用脂質(zhì)體介導的方法轉(zhuǎn)染到COS-7、HeLa和LMH細胞中，及使用磷酸鈣轉(zhuǎn)染至Huh7細胞中。平均轉(zhuǎn)染效率以相對于QIAGEN Plasmid Kit（100％）制備的DNA的轉(zhuǎn)染效率表示。

質(zhì)粒純化方法與轉(zhuǎn)染效率。

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